蔡杰;呼高伟;王乃东;邓治邦;王爱兵;杨毅;

• 1:湖南农业大学动物医学院
• 2:湖南农业大学动物医学院逆向动物疫苗研究中心

摘要(Abstract)：

从20世纪90年代开始流行到现在,全球范围内暴发的猪圆环病毒相关疾病(PCVAD)给世界各国的养猪业造成了巨大的经济损失。我国是世界的养猪大国和猪肉制品消费大国,PCVAD对我国的养猪业的发展产生了很大的影响。疫苗是预防PCVAD最直接有效的方法,文中对近年来国内外猪圆环病毒疫苗的研究进展进行综述。

关键词(KeyWords)： 猪圆环病毒;猪圆环病毒相关疾病;疫苗

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31372406)

作者(Author): 蔡杰;呼高伟;王乃东;邓治邦;王爱兵;杨毅;

Email:

DOI: 10.13326/j.jea.2016.1133

参考文献(References)：

• [1]Beach N M,Meng X J.Efficacy and future prospects of commercially available and experimental vaccines against porcine circovirus type 2(PCV2)[J].Virus Res,2012,164(1/2):33-42.
• [2]Perez-Martin E,Gomez-Sebastian S,Argilaguet J M,et al.Immunity conferred by an experimental vaccine based on the recombinant PCV2 Cap protein expressed in Trichoplusia nilarvae[J].Vaccine,2010,28(11):2340-2349.
• [3]Tu Y,Wang Y,Wang G,et al.High-level expression and immunogenicity of a porcine circovirus type 2 capsid protein through codon optimization in Pichia pastoris[J].Appl Microbiol Biotechnol,2013,97(7):2867-2875.
• [4]Zhang C,Zhu S,Wei L,et al.Recombinant Flagellin-Porcine Circovirus Type 2 Cap Fusion Protein Promotes Protective Immune Responses in Mice[J].PLo S One,2015,10(6):e0129617.
• [5]Fenaux M,Opriessnig T,Halbur P G,et al.Two amino acid mutations in the capsid protein of type 2 porcine circovirus(PCV2)enhanced PCV2 replication in vitro and attenuated the virus in vivo[J].J Virol,2004,78(24):13440-13446.
• [6]Karuppannan A K,Jong M H,Lee S H,et al.Attenuation of porcine circovirus 2 in SPF piglets by abrogation of ORF3 function[J].Virology,2009,383(2):338-347.
• [7]Fenaux M,Opriessnig T,Halbur P G,et al.A chimeric porcine circovirus(PCV)with the immunogenic capsid gene of the pathogenic PCV type 2(PCV2)cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs[J].J Virol,2004,78(12):6297-6303.
• [8]Shen H,Beach N,Huang Y,et al.Comparison of commercial and experimental porcine circovirus type 2(PCV2)vaccines using a triple challenge with PCV2,porcine reproductive and respiratory syndrome virus(PRRSV),and porcine parvovirus(PPV)[J].Vaccine,2010,28(37):5960-5966.
• [9]Gillespie J,Juhan N,Di Cristina J,et al.A genetically engineered chimeric vaccine against porcine circovirus type 2(PCV2)is genetically stable in vitro and in vivo[J].Vaccine,2008,26(33):4231-4236.
• [10]Beach N M,Ramamoorthy S,Opriessnig T,et al.Novel chimeric porcine circovirus(PCV)with the capsid gene of the emerging PCV2b subtype cloned in the genomic backbone of the non-pathogenic PCV1 is attenuated in vivo and induces protective and cross-protective immunity against PCV2b and PCV2a subtypes in pigs[J].Vaccine,2010,29(2):221-232.
• [11]Opriessnig T,Shen H,Pal N,et al.A live-attenuated chimeric porcine circovirus type 2(PCV2)vaccine is transmitted to contact pigs but is not upregulated by concurrent infection with porcine parvovirus(PPV)and porcine reproductive and respiratory syndrome virus(PRRSV)and is efficacious in a PCV2b-PRRSV-PPV challenge model[J].Clinical and Vaccine Immunology,2011,18(8):1261-1268.
• [12]Pieyro P E,Kenney S P,Gimenez-Lirola L G,et al.Expression of antigenic epitopes of porcine reproductive and respiratory syndrome virus(PRRSV)in a modified live-attenuated porcine circovirus type 2(PCV2)vaccine virus(PCV1-2a)as a potential bivalent vaccine against both PCV2 and PRRSV[J].Virus Res,2015,210:154-164.
• [13]Li W,Wang X,Bai J,et al.Construction and immunogenicity of recombinant porcine circovirus-like particles displaying somatostatin[J].Vet Microbiol,2013,163(1/2):23-32.
• [14]Zhang H,Qian P,Liu L,et al.Virus-like particles of chimeric recombinant porcine circovirus type 2 as antigen vehicle carrying foreign epitopes[J].Viruses,2014,6(12):4839-4855.
• [15]Chi J N,Wu C Y,Chien M S,et al.The preparation of porcine circovirus type 2(PCV2)virus-like particles using a recombinant pseudorabies virus and its application to vaccine development[J].J Biotechnol,2014,181:12-19.
• [16]Fan H,Pan Y,Fang L,et al.Construction and immunogenicity of recombinant pseudotype baculovirus expressing the capsid protein of porcine circovirus type 2 in mice[J].J Virol Methods,2008,150(1/2):21-26.
• [17]Xu X G,Wang Z S,Zhang Q,et al.Baculovirus as a PRRSV and PCV2 bivalent vaccine vector:baculovirus virions displaying simultaneously GP5 glycoprotein of PRRSV and capsid protein of PCV2[J].J Virol Methods,2012,179(2):359-366.
• [18]Song Y,Jin M,Zhang S,et al.Generation and immunogenicity of a recombinant pseudorabies virus expressing cap protein of porcine circovirus type 2[J].Vet Microbiol,2007,119(2/4):97-104.
• [19]Wang X,Jiang P,Li Y,et al.Protection of pigs against postweaning multisystemic wasting syndrome by a recombinant adenovirus expressing the capsid protein of porcine circovirus type 2[J].Vet Microbiol,2007,121(3/4):215-224.
• [20]Beach N M,Smith S M,Ramamoorthy S,et al.Chimeric porcine circoviruses(PCV)containing amino acid epitope tags in the C terminus of the capsid gene are infectious and elicit both anti-epitope tag antibodies and anti-PCV type 2 neutralizing antibodies in pigs[J].J Virol,2011,85(9):4591-4595.
• [21]Huang L,Lu Y,Wei Y,et al.Construction and biological characterisation of recombinant porcine circovirus type 2expressing the V5 epitope tag[J].Virus Res,2011,161(2):115-123.
• [22]Bucarey S A,Noriega J,Reyes P,et al.The optimized capsid gene of porcine circovirus type 2 expressed in yeast forms virus-like particles and elicits antibody responses in mice fed with recombinant yeast extracts[J].Vaccine,2009,27(42):5781-5790.
• [23]Patterson R,Eley T,Browne C,et al.Oral application of freeze-dried yeast particles expressing the PCV2b Cap protein on their surface induce protection to subsequent PCV2b challenge in vivo[J].Vaccine,2015,33(46):6199-6205.
• [24]Bucarey S A,Pujol M,Poblete J,et al.Chitosan microparticles loaded with yeast-derived PCV2 virus-like particles elicit antigen-specific cellular immune response in mice after oral administration[J].Virol J,2014,11(1):149.