梅花鹿茸重相关基因差异表达分析Differential Expression Analysis of the Genes Related to Velvet Antler Weight of Sika Deer
孙江洋;杨福杰;李东;宋禹昊;时坤;宗颖;李健明;杜锐;曾范利;
摘要(Abstract):
通过分析不同茸重梅花鹿茸的差异表达基因,筛选可用于区分鹿茸重量的基因。以东丰县梅花鹿为试验对象,同一饲养条件下,采集不同梅花鹿的茸血,进行高通量测序,对差异表达基因进行GO、KEGG富集分析及qRT-PCR验证。结果表明:共有84个差异基因,其中,44个基因上调,40个基因下调。通过GO分析,这些基因共富集到318个条目,其中,分子功能118个、生物过程138个和细胞组分62个;通过KEGG分析,富集于细胞黏附分子(CAMs)、抗原加工递呈、Ⅰ型糖尿病等106条通路。通过P值筛选出8个基因进行qRT-PCR验证,趋势与测序数据相符,TRPC6基因在高产组的相对表达量高于低产组(P<0.01),有望成为筛选种公鹿的候选基因,为后续梅花鹿的良种选育提供理论基础。
关键词(KeyWords): 梅花鹿茸;转录组;差异表达基因;qRT-PCR
基金项目(Foundation): 科技部国家重点研发计划项目(2018YFC1706601)
作者(Authors): 孙江洋;杨福杰;李东;宋禹昊;时坤;宗颖;李健明;杜锐;曾范利;
DOI: 10.13326/j.jea.2021.1581
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