驯鹿生长素Ghrelin cDNA的克隆cDNA Cloning of Reindeer Ghrelin
杨银凤,朱雪敏,余兴帮,赵艳芳
摘要(Abstract)：
从驯鹿皱胃组织中提取总RNA,根据已发表的驯鹿生长素Ghrelin基因序列设计并合成引物,通过反转录-聚合酶链式反应(RT-PCR)进行cDNA扩增,获得了300 bp的片段,重组到pBlueselect T载体,经限制性内切酶谱分析和DNA序列测定分析,确认PCR产物为Ghrelin cDNA,为进一步研究Chrelin在驯鹿体内的分布及营养因素等对其基因表达的影响奠定基础。
关键词(KeyWords)： 生长素;驯鹿;cDNA
基金项目(Foundation): 内蒙古农业大学博士启动基金资助项目(BJ04-2)
作者(Author): 杨银凤,朱雪敏,余兴帮,赵艳芳
DOI: 10.13326/j.jea.2007.04.009
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