一株不发酵乳糖致病性大肠杆菌的分离与鉴定Isolation and Identification of a Non-Lactose-Fermenting Pathogenic Escherichia coli from Guinea Fowl
古飞霞,陈锦均,刘容珍,胡婉仪,温建成
摘要(Abstract):
从广东某珍珠鸡场发病死亡的初产珍珠鸡肝脏中分离到一株细菌,经培养特性与生化特性观察,以致病性试验和血清型鉴定,结合其16S rRNA序列分析,判定其为大肠杆菌。这是一株不发酵乳糖的致病性大肠杆菌。
关键词(KeyWords): 大肠杆菌;特性;16S rRNA序列;珍珠鸡
基金项目(Foundation):
作者(Author): 古飞霞,陈锦均,刘容珍,胡婉仪,温建成
DOI: 10.13326/j.jea.2009.04.004
参考文献(References):
- [1]Jeffrey L Watts,Thomas R Shryock,Michael Apley,et al.Perfor-mance standards for antimicrobial disk and dilution susceptibilitytests for bacteria isolated from animals[M].3rd ed.Wayne Penn-sylvania,USA:Standards Institute,2008:13-89.
- [2]吕俊超,李轩,韩茵,等.养殖大菱鲆中牙鲆肠弧菌的分离鉴定及组织病理学[J].水产学报,2009,33(2):311-317.
- [3]何金林,夏子森,张瑗.10株误诊为志贺氏菌的低活性大肠埃希氏菌的细菌学分析[J].浙江预防医学,1992(4):36.
- [4]周海洋.珍珠鸡大肠杆菌病的诊断与防治[J].吉林畜牧兽医,2006(6):47.
- [5]朱道荣.珍珠鸡大肠杆菌性眼炎的诊治[J].广西畜牧兽医,2003,19(6):262-263.
- [6]成大荣,朱善元.不发酵或迟发酵乳糖型禽大肠杆菌的分离鉴定[J].动物医学进展,2009,30(5):28-31.
- [7]Dhillon A S,Jack O K.Two outbreaks of colibacillosis in commer-cial caged layers[J].Avian Dis,1996,40:742-746.
- [8]Usman B A,Diarra S S.Prevalent diseases and mortality in eggtype layers:An overview[J].International Journal of Poultry Sci-ence,2008,7(4):304-310.
- [9]Zanella A,Alborali G L,Bardotti M,et al.Severe Escherichiacoli O111 septicaemia and polyserositis in hens at the start of lay[J].Avian Pathology,2000,29:311-317.
- [10]黎杰虹,陆英杰,徐维成.佛山地区禽源大肠杆菌血清型研究[J].佛山科学技术学院学报:自然科学版,2003,21(1):75-77.
- [11]王林川,王建强,吴福东,等.广东部分地区水禽致病性大肠杆菌血清型调查[J].养禽与禽病防治,1998(11):6-7.
- [12]Wooley R E,Gibbs P S,Brown T P,et al.Chicken embryolethality assay for determining the virulence of avian Escherichiacoli isolates[J].Avian Dis,2000,44:318-324.
- [13]马迪根,马丁克.微生物生物学[M].杨文博,译.北京:科学出版社,2001:767-763.
- [14]Timothy J J,Subhashinie K,Yvonne W,et al.The genome se-quence of avian pathogenic Escherichia coli strain O1:K1:H7shares strong similarities with human extraintestinal pathogenic E.coli Genomes[J].Journal of Bacteriology,2007,189(8):3228-3236.