刘忠军,卢天凤

• 1:吉林农业大学中药材学院

摘要(Abstract)：

选用瘤胃尚未发育的初生小尾寒羊羔羊及梅花仔鹿各4只,人工哺乳,随牛奶分别饲喂给羔羊和仔鹿5个水平的核酸。羔羊尿中嘌呤衍生物的总量(Y,mmol/d)随着摄入嘌呤量(X,mmol/d)的增加而增加。其线性方程为:Y=0.752+0.662X(r=0.995,P<0.001)。仔鹿尿中嘌呤衍生物的总量(Y,mmol/d)随着摄入嘌呤量(X,mmol/d)的增加也明显增加,线性方程为:Y=0.559+0.326X(r=0.990,P=0.001)。利用紫外分光光度计测定的羔羊尿中尿酸当量值与嘌呤摄入量呈强正相关(r=0.994,P<0.01)。由此推算出利用尿中尿酸当量值来估测羊小肠微生物氮流量(Y,g/d)的方程为:Y=0.417+0.396X;推算出的利用尿中尿酸当量值估测鹿小肠微生物氮流量(Y,g/d)的方程为:Y=-4.137+1.245X。X(mmol/d)为测得的羊或鹿尿中尿酸当量值。

关键词(KeyWords)： 反刍动物;微生物蛋白流量;尿酸当量

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 刘忠军,卢天凤

DOI: 10.13326/j.jea.2012.01.005

参考文献(References)：

• [1]Siddons R C,Beever D E,Nolan J V.A comparison of methods forthe estimation of microbial nitrogen induodenal digesta of sheep[J].Br J Nutr,1982,48:377-389.
• [2]Storm E,Brown D S,rskov E R.The nutritive value of rumen mi-cro-organisms in ruminants.3.The digestion of microbial aminoand nucleic acids in,and loses of endogenous nitrogen from smallintestine of sheep[J].Br J Nutr,1983,50:479-485.
• [3]Liu Zhongjun.Microbial N Flow to the Duodenum of Sheep Esti-mated Using Excretion of Purine Derivative Excretion in the Urine[D].Australia:The University of Queensland,2000.
• [4]Mathison G W,Milligan L P.Nitrogen metabolism in sheep[J].Brit J Nutr,1971,25:351-366.
• [5]Mathison G W,Milligan L P.Nitrogen metabolism in sheep[J].BritJ Nutr,1971,25:351-366.
• [6]Pilgrim A F,Gray F V,Weller R A,et al.Synthesis of microbialprotein from ammonia in the sheep′s rumen and the proportion ofdietary nitrogen converted into microbial nitrogen[J].Brit J Nutr,1970,24:589-598.
• [7]Beever D E,Harrison D G,Thomson D J,et al.A method for theestimation of dietary and microbial protein in duodenum digesta ofruminants[J].Br J Nutr,1974,32:99-112.
• [8]Walker D J,Nader C J.Measurement in vivo of rumen microbialprotein synthesis[J].Aust J Agric Res,1975,26:689-698.
• [9]Ling J R,Buttery P J.The simultaneous use of ribonucleicacid,35S,2,6-diaminopimelic acid and 2-aminoethylphosphonicacid as markers of microbial nitrogen entering the duodenum ofsheep[J].Br J Nutr,1978,39:165-179.
• [10]Mathers J C,Miller E L.A simple procedure using 35S incorpora-tion for the measurement of microbial and undegraded food proteinin ruminant digesta[J].Br J Nutr,1980,43:503-514.
• [11]Bucholtz H F,Bergen W G.Microbial phospholipid synthesis asamarker for microbial protein synthesis in the rumen[J].Appl Mi-crobiol,1973,25(4):504-513.
• [12]Van Nevel C J,Demeyer D I.Determination of rumen microbialgrowth in vitro from 32P-labelled phosphate incorporation[J].Br JNutr,1977,38:101-114.
• [13]Czerkawski J W.Methods for determining 2-6-diaminopimelic acidand 2-aminoethylphosphonic acid in gut contents[J].J Food A-gric,1974,25:45-55.
• [14]Illg D J,Stern M D.In vitro and in vivo comparisons of di-aminopimelic acid and purines for estimating protein synthesis inthe rumen[J].Anim Feed Sci Tech,1994,48:49-55.
• [15]Forsberg C W,Lam K.Use of adenosine 5′-triphosphate as an in-dicator of the microbiota biomass in rumen contents[J].Appl En-viromental Microbiol,1977,33:528-537.
• [16]Evans R A,Axford R F E,Offer N W.A method for estimating thequantities of microbial and dietary proteins flowing in the duodenaldigesta of ruminants[J].Proc Nutr Soc,1975,34:65A
• [17]Stern E,Hoover W H.Methods for determining and factors affect-ing rumen microbial protein synthesis:A review[J].J Anim Sci,1979,49(6):1590-1603.
• [18]Smith R H.Nitrogen metabolism in the rumen and the compositionand nutritive value of nitrogen compounds entering the duodenum[C]∥McDonald I W,Warner A C I.Digestion and Metabolism inruminant.Armidale,NSW,Australia:The Univ.of New EnglandPublishing Unit,1975.
• [19]McRae J C,Wilson S.The effects of various forms of gastro intesti-nal cannulation on digestive measurements in sheep[J].Br J Nutr,1977,38:65-74.
• [20]Wenham G.Effects of cannulation on intestinal motility[J].AnnRech Vet,1979,10:157-166.
• [21]Topps J H,Elliot R C.Relationships between concentrations of ru-minal nucleic acid and excretion of purine derivatives by sheep[J].Nature(London),1965,205:498-499.
• [22]Rys R,Antoniewicz J,Maciejewicz J.Allantoin in urine as an in-dex of microbial protein in the rumen[M].Symposium,Vienna,Austria:Tracer Studies on Non-Protein Nitrogen for Ruminants,1975.
• [23]Chen X B,Hovell F D DeB,rskov E R,et al.Excretion ofpurine derivatives by ruminants:Effects of exogenous nucleic acidsupply on purine derivative excretion by sheep[J].Br J Nutr,1990,63:131-142.
• [24]沈同,王镜岩.生物化学[M].北京:高等教育出版社,1991:297-301.
• [25]杨安钢,毛积分,药立波.生物化学与分子生物学试验技术[M].北京:高等教育出版社,2001:101-105.
• [26]Balcells J,Guada J A,Peiro J M,et al.Short communication:Simultaneous determination of allantoin and oxypurines in biologi-cal fluids by high-performance liquid chromatography[J].J Chro-matography,1992,575:153-157.
• [27]张晓勇,刘晓,刘忠军.乳酸菌对绵羊消化率及尿酸当量的影响[J].饲料研究,2011(1):21-23.