水貂多巴胺受体D2基因的克隆测序及外显子区多态性检测Cloning,Sequencing and Detecting Exon Polymorphism of Dopamine Receptor D2 Gene in Mink
林宁,白秀娟
摘要(Abstract)：
以多巴胺受体D2基因(DRD2)作为候选基因,分析该基因对水貂自咬行为的影响。本试验采用聚合酶链式反应方法,首次从美洲黑貂脚部肌肉组织扩增出多巴胺受体D2基因的部分序列片段,并克隆测序,将该序列提交到Gene Bank上,已得到序列号为EU085471。通过序列比较,在第四外显子区域未发现碱基突变,在第5外显子区域发现在41位点处发生了(T→C)转换,采用单链构象多态性(PCR-SSCP)方法进行了多态性检测,结果表明:在不同个体中未检测到基因多态性。
关键词(KeyWords)： 美洲黑貂;多巴胺受体D2基因;克隆测序;PCR-SSCP
基金项目(Foundation):
作者(Author): 林宁,白秀娟
DOI: 10.13326/j.jea.2008.01.012
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