詹崇伟,曾祥伟,邢明伟,汤超文,于洪

• 1:东北林业大学野生动物资源学院

摘要(Abstract)：

根据GenBank上已发表的犬科动物的白细胞介素-2(IL-2)基因序列,在开放阅读框架上下游的保守区域设计了1对引物。无菌采取貉血,使用淋巴细胞分离液分离外周血液中的淋巴细胞,加入ConA,于二氧化碳培养箱中培养48 h后收集培养细胞。以培养细胞中提取的总RNA为模板,应用RT-PCR方法,扩增出貉的IL-2基因。分离纯化片段,连接T载体转化大肠杆菌并测序。结果表明:IL-2基因开放阅读框架全长486 bp,编码155个氨基酸。该序列与犬、狐等犬科动物的IL-2基因同源关系最近,与大熊猫、家猫的IL-2基因同源关系相对较近,与人、马、牛、绵羊的IL-2基因有一定的遗传距离,与禽类鸡的IL-2基因同源关系最远。

关键词(KeyWords)： 貉;白细胞介素-2;克隆;进化关系

Abstract:

Keywords:

基金项目(Foundation): 东北林业大学大学生创新实验项目(2008005);; 黑龙江省青年科技专项资金资助项目(QC07C33);; 黑龙江省博士后科研启动资金资助项目(LBH-Q08150)

作者(Author): 詹崇伟,曾祥伟,邢明伟,汤超文,于洪

DOI: 10.13326/j.jea.2009.04.006

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