狍Sry基因PCR扩增的初步研究PCR Amplification of the Sry Gene of Roe Deer(Capreolus capreolus)
李平,白秀娟
摘要(Abstract)：
为研究狍性别决定机制,采用聚合酶链式反应(PCR)技术对野生狍(Capreoluscapreolus)(n♀=2,n♂=2)Sry基因(哺乳动物Y染色体DNA雄性特异区)进行扩增。根据人的SRY基因核心序列设计合成了1对引物1,2。结果在野生狍雄性个体中扩增出1条带,大小约为220bp,而在雌性个体中未见扩增带,表明了Sry基因的性别特异性,为探讨野生狍的性别决定机制提供了分子资料。
关键词(KeyWords)： 狍;Sry基因;聚合酶链式反应技术
基金项目(Foundation):
作者(Author): 李平,白秀娟
DOI: 10.13326/j.jea.2005.02.014
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