李秀峰,肖向红,柴龙会,张晶钰

• 1:东北林业大学野生动物资源学院

摘要(Abstract)：

采用葡萄糖氧化酶-过氧化物酶法、蒽酮比色定糖法分别检测低温胁迫下东北林蛙血糖和肝糖原、肌糖原的含量,并通过6-磷酸葡萄糖脱氢酶偶联比色法、乳酸脱氢酶偶联比色法分别检测低温胁迫下东北林蛙肝脏、肌肉糖原磷酸化酶(Glycogen phosphorylase,GP)和肝脏、肾脏磷酸烯醇式丙酮酸羧激酶(PEPCK)的比活性,以探讨低温对东北林蛙GP、PEPCK比活性的影响。结果表明:随着环境温度降低,东北林蛙血糖水平逐步升高,糖原含量逐步降低,与对照组相比,各低温组血糖升高极显著(P<0.01),而肝糖原和肌糖原含量降低极显著(P<0.01);与对照组相比,0℃组、-1℃组肝脏、肌肉组织GP比活性升高极显著(P<0.01),且肝脏、肾脏PEPCK比活性升高极显著(P<0.01)。说明低温胁迫促使东北林蛙糖原含量降低,糖原分解和糖异生限速酶比活性升高,进而血糖含量升高,提示血糖作为抗冻保护剂在东北林蛙冬眠中发挥重要作用。

关键词(KeyWords)： 东北林蛙;冬眠;血糖;糖原;糖原磷酸化酶;磷酸烯醇式丙酮酸羧激酶

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30870301);; 黑龙江省自然科学基金重点项目(ZJN0604-02)

作者(Author): 李秀峰,肖向红,柴龙会,张晶钰

DOI: 10.13326/j.jea.2012.02.015

参考文献(References)：

• [1]张强,谢锋,江建平,等.冷血脊椎动物冷适应研究[J].四川动物,2006,25(2):433-438.
• [2]Storey J M,Storey K B.Triggering of cryoprotectant synthesis by the initiation of ice nucleation in the freeze tolerant frogRana syl-vatica[J].Comp Physiological Society(B),1985,156:191-195.
• [3]Joshua R Edwards,Karen L Koster,David L Swanson.Time course for cryoprotectant synthesis in the freeze-tolerant chorusfrog,Pseudacris triseriata[J].Comparative Biochemistry and Physiology Part A,2000,125:367-375.
• [4]Xiao Xianghong,Zheng Dong,Yang Cuijun,et al.Survival and Metabolic Responses to FreezingTemperature in the Northeast For-est FrogRana dybowskii[J].Asiatic Herpetological Research,2008,11:147-152.
• [5]Bergmeyer HU.Methods of Enzymatic Analysis[M].Weinheim:Verlag Chemie,1983,2:293-295.
• [6]龚明.脂联素对正常大鼠肝细胞GK与PEPCK酶活性与mRNA表达的影响[D].重庆:重庆医科大学,2005.
• [7]Storey K B.Reptile freeze tolerance:metabolism and gene expres-sion[J].Cryobiology,2006,52(1):1-16.
• [8]Irwin JT,Costanzo J P,Lee R E Jr.Post freeze reduction of loco-motor endurance in the freeze tolerant wood frog,Rana sylvatica[J].Physiol Biochem Zool,2003,76(3):331-338.
• [9]Sergio Polakof,Thomas PMommsen,JoséL Soengas.Glucosensing and glucose homeostasis:From fish to mammals[J].Comparative Biochemistry and Physiology(Part B),2011,160:123-149.
• [10]Steiner AA,Petenusci S O,Brentegani L G,et al.The impor-tance ofglucose forthe freezingtolerance/intolerance ofthe anuran amphibiansRana catesbeianaandBufo paracnemis[J].Rev Bras Biol,2000,60(2):321-328.
• [11]Jennifer L Jenkins,David L Swanson.Liver glycogen,glucose mobilization and freezing survival in chorusfrogs,Pseudacris trise-riata[J].Journal of Thermal Biology,2005,30:485-494.
• [12]Paul H Donohoe,Robert G Boutilier.The protective effects of metabolic rate depression in hypoxic cold submerged frogs[J].Respiration Physiology,1998,111(3):325-336.
• [13]Ziegler R,Ashida M,Fallon AM,et al.Regulation of glycogen phosphorylase in fat body ofCecropiasilkmoth pupae[J].J Comp Physiol,1979,131:321-332.
• [14]Storey K B,Storey JM.Biochemical adaptation for freezingtoler-ance in the wood frog,Rana sylvatica[J].J Comp Physiol B,1984,155:29-36.
• [15]Benson E,Fuller B,Lane N.Life in the Frozen State[M].Boca Raton:CRC Press,2004:243-274.
• [16]Daria Dziewulska-Szwajkowska,Magorzata oziska-Gabska,An-drzej Dugaj.Rana esculentaL.Liver Fru-1.6-P2ase and G-6-Pase activity and Fru-2.6-P2concentration after acclimation at5 and25℃[J].Comparative Biochemistry and Physiology Part A:Physiology,1997,118(3):745-751.