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为探究过氧化物酶增殖物激活受体γ(Peroxisome proliferator-activated receptors γ,PPARγ)在性成熟前公羊生殖器官中的表达情况，以性成熟前雄性绵羊为研究对象，测量睾丸、附睾和输精管等表型参数，采用免疫组织化学染色、实时荧光定量PCR和免疫印迹法，检测PPARγ在性成熟前公羊生殖器官中的表达及定位。结果表明：大睾丸组睾丸质量、纵轴长、输精管长、横轴长和附睾质量均显著高于小睾丸组（P<0.05）;PPARγ蛋白在睾丸支持细胞、间质细胞、各级生精细胞，以及附睾和输精管的上皮细胞、精子、纤毛中均有表达；大睾丸组PPARγ mRNA和蛋白在睾丸、输精管、附睾头、附睾体、附睾尾中的相对表达量均高于小睾丸组。推测PPARγ可能与睾丸发育有关，是影响绵羊繁殖性能的关键因子，研究结果为揭示精子发生发育过程提供了新思路。
Abstract：In order to explore the expression of peroxidase proliferator-activated receptor γ(Peroxisome proliferator-activated receptors γ,PPARγ) in the reproductive organs of rams before sexual maturity, prepubertal male sheep were used as the research subjects. And the parameters of testes, epididymis and vas deferens were measured. Immunohistochemistry, fluorescence quantitative PCR and immunoblotting were used to detect the expression and localization of PPARγ in the reproductive organs of the male sheep before sexual maturity. The results showed that the testicular weight, longitudinal axis length, vas deferens length, transverse axis length and epididymal weight in the large testicular group were significantly higher than those in the small testicular group(P<0.05). PPARγ protein was expressed in sertoli cells, mesenchymal cells, spermatogenic cells at all levels, and epididymis and vas deferens of epidimelial cells, spermatozoa and cilia. The expression levels of PPARγ mRNA and protein in the testis, vas deferens, caput epididymidis, corpus epididymidis and cauda epididymidis in the large testis group were higher than those in the small testis group. It is speculated that PPARγ may be related to testicular development and is a key factor affecting the reproductive performance of sheep, and provide a new path for revealing the process of spermatogenesis and development.
[1]Endo T, Mikedis M M, Nicholls P K, et al. Retinoic acid and germ cell development in the ovary and testis[J]. Biomolecules, 2019, 9(12):775.
[2]Gewiss R, Topping T, Griswold M D. Cycles, waves, and pulses:Retinoic acid and the organization of spermatogenesis[J].Andrology, 2020, 8(4):892-897.
[3]Ma T, Xia Y H, Wang B, et al. Environmentally relevant perinatal exposure to DBP accelerated spermatogenesis by promoting the glycolipid metabolism of Sertoli cells in male offspring mice[J]. Toxicology Letters, 2023, 377:16-28.
[4]Santoro M, De Amicis F, Aquila S, et al. Peroxisome proliferator-activated receptor gamma expression along the male genital system and its role in male fertility[J]. Human Reproduction, 2020, 35(9):2072-2085.
[5]Kang Z L, Qiao N, Liu G Y, et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells[J]. Toxicology in Vitro,2019, 61:104639.
[6]Gorga A, Rindone G M, Regueira M, et al. PPARγ activation regulates lipid droplet formation and lactate production in rat Sertoli cells[J]. Cell and Tissue Research, 2017, 369(3):611-624.
[7]张馨元，孙义乐，宋军，等.家畜性别控制技术研究进展[J].经济动物学报，2025, 29(3):200-207.
[8]张达，吴秀菊，袁崇善，等.丝胶蛋白对牛精液冷冻效果的影响[J].吉林农业大学学报，2022, 44(1):86-91.
[9]Shi J F, Li Y K, Ren K, et al. Characterization of cholesterol metabolism in Sertoli cells and spermatogenesis(Review)[J].Molecular Medicine Reports, 2018, 17(1):705-713.
[10]赵彤，张祎俐，石鸿娇，等.超重、肥胖男童阴茎睾丸发育的相关性研究[J].医学研究杂志，2023, 52(3):44-48.
[11]夏雨婷，万永杰，姜志洋，等.日粮添加酵母硒对夏季湖羊血液指标和睾丸发育及基因表达的影响[J].南京农业大学学报，2022, 45(4):752-760.
[12]Zhang Y, Li X Y, Jing L, et al. Decabromodiphenyl ether induces male reproductive toxicity by activating mitochondrial apoptotic pathway through glycolipid metabolism dysbiosis[J].Chemosphere, 2021, 285:131512.
[13]赵啸宇，朱琼，恽朝晖，等.肥胖因子三丁基锡影响细胞代谢功能的研究进展[J].中国细胞生物学学报，2020, 42(9):1650-1655.
[14]Jin J, Huangfu B X, Xing F G, et al. Combined exposure to deoxynivalenol facilitates lipid metabolism disorder in highfat-diet-induced obesity mice[J]. Environment International,2023, 182:108345.
[15]杜炜.绵羊附睾头、体、尾上皮细胞的生物学特性及基因表达[D].呼和浩特：内蒙古农业大学，2021.
[16]Khamehiee N, Jazireian P, Ebrahimi B, et al. Paternal transfatty acid and vitamin E diet affects rat offspring′s semen quality and PPARs expression[J]. Andrologia, 2021, 53(7):e14082.
[17]Xia Q, Di R, He X Y, et al. Expression analysis of DIO2,EYA3, KISS1 and GPR54 genes in year-round estrous and seasonally estrous rams[J]. Archives Animal Breeding,2020, 63(2):451-460.
[18]Passaponti S, Signorini C, Collodel G, et al. Expression and immunolocalisation of PPARγ in reproductive tissues of rabbits fed n-3 PUFA-enriched diets[J]. Journal of Mens Health,2022, 18(3):72.
[19]卿恩华.饲养方式对不同品种公鹅生殖器官发育的影响及其调控机制研究[D].雅安：四川农业大学，2023.
[20]要荣宇.影响湖羊睾丸发育的差异代谢物筛选及机制研究[D].兰州：兰州大学，2022.
[21]Montes-Garrido R, Anel-Lopez L, Riesco M F, et al. Does size matter? Testicular volume and its predictive ability of sperm production in rams[J]. Animals, 2023, 13(20):3204.
[22]Yao P L, Chen L P, Hess R A, et al. Peroxisome proliferator-activated receptor-D(PPARD)coordinates mouse spermatogenesis by modulating extracellular signal-regulated kinase(ERK)-dependent signaling[J]. The Journal of Biological Chemistry, 2015, 290(38):23416-23431.
[23]高佃军，高波，祝增军，等.邻苯二甲酸二酯与特发性少弱精子症关系的探讨[J].中华男科学杂志，2014, 20(8):702-705.
[24]Pourazadi L, Sharafi M, Torshizi M A K, et al. Modulatory effects of pioglitazone as a ligand for the peroxisome proliferator-activated receptor on semen quality and fertility potential of broiler breeder roosters[J]. Poultry Science, 2022, 101(5):101795.
基本信息:
DOI：10.13326/j.jea.2026.1911
中图分类号:S826
引用信息:
[1]孙鑫铭,柳杭,孙丽敏,等.PPARγ在性成熟前公羊生殖器官的表达差异分析[J].经济动物学报,2026,30(01):43-48.DOI:10.13326/j.jea.2026.1911.
基金信息:
国家重点研发计划项目（2021YFF1000702）; 吉林省科技发展计划项目（202000402039NC）; 吉林省自然科学基金项目（20220101342JC）