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姜黄素作为一种来源于植物的天然多酚类物质，具有抗氧化及抗炎等作用，已广泛应用于人类保健以及畜禽生产中，在宠物日粮中的应用也逐渐增多。为探究姜黄素对猫肾细胞的影响及其抗氧化作用，以姜黄素对自由基的清除率为指标，分析姜黄素的抗氧化活性；用H2O2建立猫肾细胞损伤模型，探索姜黄素对猫肾细胞氧化应激损伤的作用，利用转录组学分析姜黄素影响猫肾细胞的基因功能通路。结果表明：姜黄素对DPPH自由基的清除能力具有剂量依赖性，姜黄素浓度为100μmol/L时，清除率>50%。此外，与阴性对照组相比，姜黄素可增强猫肾细胞过氧化氢酶、总超氧化物歧化酶以及谷胱甘肽过氧化物酶的活性，且对H2O2引起的猫肾细胞损伤具有一定的保护作用。转录组测序发现，姜黄素可引起猫肾细胞基因差异转录，参与生物过程、细胞组成、分子功能等。姜黄素对猫肾细胞存在一定的抗氧化作用，研究结果为姜黄素在宠物食品中的应用提供依据。
Abstract：Curcumin, a naturally occurring polyphenolic compound extracted from plants, is demonstrated a wide array of physiological activities, including antioxidant, anti-inflammatory, and anticancer effects. Its multifaceted properties have facilitated its application in human healthcare as well as in livestock and poultry production, with an increasing incorporation into formulations for pet food. This study seeks to investigate the influence of curcumin on feline renal cells and its antioxidant activity. The chemical antioxidant capacity of curcumin was evaluated by measuring free radical scavenging rates; subsequently, feline kidney cells were employed as a model for renal injury induced by H2O2 to further clarify the protective effects of curcumin against oxidative stress-related damage. Additionally, transcriptomic analysis was conducted to examine the gene functional pathways modulated by curcumin in F81 cells. The results indicated that curcumin exhibited a dose-dependent scavenging effect on DPPH free radicals, achieving a 50% scavenging rate at a concentration of 100 μmol/L. Moreover, curcumin prompted F81 cells to elevate the activity levels of various antioxidant enzymes such as catalase(P<0.001), superoxide dismutase(P<0.05), and glutathione(P<0.01), thereby providing substantial protection against H2O2-induced cellular damage. Through transcriptome sequencing, it was found that curcumin can induce differential transcription of genes in cat kidney cells, participate in biological response processes, cell composition, immune pathways, etc. This study demonstrated that curcumin exhibited a certain level of antioxidant activity for F81 cells, providing a certain theoretical basis for the application of curcumin in pet food development.
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基本信息:
DOI：10.13326/j.jea.2025.2034
中图分类号:S829.3
引用信息:
[1]李丹丹,丁方舟,曹佳等.姜黄素对猫肾细胞氧化损伤的保护作用[J].经济动物学报,2025,29(02):159-168.DOI:10.13326/j.jea.2025.2034.
基金信息:
浙江省农业科学院横向合作项目（2024R22B88D01）