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为探究内蒙古地区绵羊携带病毒情况，采集内蒙古某杜泊羊规模化养殖场的30只健康成年羊与10只腹泻羔羊的鼻咽拭子和肛拭子，通过宏转录组法和多重替代扩增法进行全谱病毒组学分析。结果表明：共注释到12个哺乳动物病毒科，对星状病毒、肠道病毒、口疮病毒与轮状病毒进行遗传进化分析，发现星状病毒与已知病毒遗传差异较大，为新病毒种；肠道病毒、口疮病毒和轮状病毒与参考毒株差异较小；PCR检测结果表明，该地区绵羊携带病毒具有多样性，且存在混合感染情况，初步获得了该养殖场病毒本底数据。研究结果丰富了该地区杜泊羊携带病毒的多样性数据，为病毒性疾病的预防提供参考。
Abstract：To investigate the virus carrying situation of sheep in Inner Mongolia region, nasopharyngeal swab and anal swab samples of 30 adult sheep and 10 diarrheic lambs from a large-scale Dupo sheep farm in Inner Mongolia were analyzed for full-spectrum virological analysis using a combination of meta-transcriptomic and multiple displacement amplification methods, which resulted in the identification of a total of 12 mammalian virus families. Genetic evolutionary analyses of astroviruses, enteroviruses, stomatitis viruses and rotaviruses revealed that astroviruses were genetically different from existing known viruses and were new viral species, while enteroviruses, stomatitis viruses and rotaviruses showed less differences with the reference strains. The PCR detection revealed the prevalence of the viruses carried by sheep in the farm and exhibited mixed infections, which obtained the background data of the viral diseases present in this farm. The research results enriched the data of the diversity of viruses carried by Dupo sheep in the region, laying a foundation, which helps understand the diversity of viruses carried by Dupo sheep and prevent viral infectious diseases.
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基本信息:
DOI：10.13326/j.jea.2025.1886
中图分类号:S858.26
引用信息:
[1]王慧羽,特木尔巴根,孙悦,等.内蒙古杜泊羊鼻咽拭子和肛拭子样本的全病毒组分析[J].经济动物学报,2025,29(06):455-462.DOI:10.13326/j.jea.2025.1886.
基金信息:
国家自然科学基金项目（32130104）